A study into the role of H3.3 Serine 31 phosphorylation in chromatin regulation — ASN Events
  1. Schedule
  2. Poster Session 2
  3. A study into the role of H3.3 Serine 31 phosphorylation in chromatin regulation

A study into the role of H3.3 Serine 31 phosphorylation in chromatin regulation (#231)

Vinod Benjamin 1 , Hsiao PJ Voon 1 , Maheshi Udugama 1 , Lyn F Chan 1 , Fiona TM Chang 1 , Jeffery R Mann 1 , Lee H Wong 1
  1. Monash University, Clayton, VIC, Australia

Histone H3.3 is a H3 variant that is encoded by 2 genes, H3F3A and H3F3B. Unlike the canonical H3.1 and H3.2, H3.3 has a Serine at residue 31 which is evolutionarily conserved and known to be phosphorylated during mitosis. The phosphorylated serine 31 on H3.3 (H3.3S31Ph) is found predominantly at the pericentric heterochromatin and telomeres in mammalian cells.

In this study, we aim to define and characterise the function and regulation of H3.3S31Ph by using the mouse embryonic stem cells (ESCs) as a model system. To accomplish this, ES mutants carrying Serine 31 to Alanine (phosphonull) and Glutamic acid (phosphomimic) mutations were generated, respectively. Here, we will present data on the impact of both the loss and gain of H3.3S31Ph on cellular growth, gene transcription activity and genome stability in the mutant cells.

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