State of the art of single cell transcriptomics. — ASN Events

State of the art of single cell transcriptomics. (#268)

Lavinia Gordon 1 , Milica Ng 2 , Nicholas Wilson 3 , Mark Biondo 3 , Matt Tinning 1
  1. AGRF, Parkville, VIC, Australia
  2. Bioinformatics, CSL Limited, Parkville, Victoria, Australia
  3. Translational Research, CSL Limited, Parkville, VIC, Australia

Describing the transcriptome of individual cells is vital to understanding intricate biological systems. The 10X Genomics Chromium System is a droplet-based system that enables 3’ mRNA counting of up to tens of thousands of single cells per sample. Cell encapsulation in droplets takes place in less than 7 minutes, with ~50% cell capture efficiency, and has throughput of up to 8 samples at a time. Here we describe the profiling of peripheral blood mononuclear cells (PBMCs) to demonstrate the ease of the workflow from sample preparation through to data analysis.  The Chromium data is compared to bulk RNAseq data to highlight the differences in the transcriptome captured between the two systems.

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